TY - JOUR A2 - 迪特里希，克里斯托夫G. AU - 刘，陶丽AU - 张，李娜AU - 谷岳宇AU - 林美桂盟 - 谢君AU - 晨，俞玲AU - 刘，贾辉AU - 吴昕林AU - 莫穗林PY - 2020 DA - 2020年3月10日TI - 对人肝癌裸鼠移植丹参酮IIA加上阿霉素的协同抗肿瘤作用BALB /c裸鼠及其对细胞色素P450 CYP3A4影响体内SP - 6231751 VL - 2020 AB - 目的。肝癌是最常见的疾病是严重威胁人类生命和健康的一个。在这项研究中，我们评估了对人肝癌阿霉素（ADM）联合丹参酮IIA（丹参酮IIA）的抑制作用，并开发了一个平台，以评估功能，如果中国中药成分与化疗药物联合应用具有协同抗肿瘤作用 体内。 方法。人肝癌肝癌细胞在裸鼠建立动物模型。Mice were divided into model control group, Tan IIA group, ADM group, and Tan IIA + ADM group. The changes from general condition, weight, tumor volume, and inhibition rate were observed. The data were gathered from serum AST level and histopathological changes. The content and activity of cytochrome P450 were determined by spectrophotometric analysis. CYP3A4 protein expression was analyzed by western blotting. The binding model crystal structure of Tan IIA and ADM with pregnane X receptor (PXR) was evaluated by Discovery Studio 2.1. 结果。丹参酮IIA与ADM的组合可以通过减轻ADM毒性，降低肿瘤体积，减少血清AST水平，以及提高在荷瘤小鼠衬垫病理切片提高生活质量。丹参酮IIA，ADM和共处理的抑制率分别为32.77％，60.96％，73.18和％。谭IIA组显著增强色素b5，P450的内容，和红霉素 ñ-demethylase活动。CYP3A4 protein expression was enhanced obviously by the Tan IIA + ADM group. Virtual molecular docking showed that both Tan IIA and ADM could be stably docked with the same binding site of PXR but different interactions. 结论。丹参酮IIA联合ADM能提高荷瘤小鼠的生存质量，增强抗肿瘤作用。谭IIA族增加细胞色素P450的酶和酶活性的浓度。联合丹参酮IIA与ADM能上调CYP3A4蛋白的表达，并与虚拟对接蛋白质PXR相关的互动。SN - 2356-6752 UR - https://doi.org/10.1155/2020/6231751 DO - 10.1155 /六百二十三万一千七百五十一分之二千〇二十JF - Hindawi出版KW - - ER在医学PB进展 -