TY -的A2 Manaenko Anatol AU - Lan,瑞盟——张,永盟,吴道盟- Ma, Yun-Zhi AU - Wang Bao-Qi盟——郑Hai-Zhong AU - Li Ya-Na AU - Wang Yan盟——顾Chun-Qing盟——吴Ji-Tao PY - 2018 DA - 2018/06/19 TI - Xiao-Xu-Ming汤减少Mitophagy激活线粒体功能和改善脑缺血和再灌注损伤SP - 4147502六世- 2018 AB -我们调查是否Xiao-Xu-Ming汤减少Mitophagy激活并保持在脑缺血再灌注损伤的线粒体功能。大鼠随机分为5组:骗局,缺血再灌注(IR),红外+ XXMD(60克/公斤/天)(XXMD60),红外+环孢菌素A(10毫克/公斤/天)(CsA)和红外+车辆(车辆)。局灶性脑缺血和再灌注模型引起的大脑中动脉阻塞(MCAO)。脑梗塞区域由氯化三苯四唑染色法测定。脑缺血性损伤评估通过苏木精和伊红染色(他)和尼氏小染色。线粒体的超微结构特征和mitophagy半影的缺血皮层由透射电子显微镜观察。Mitophagy被免疫荧光标记和LC3B VDAC1检测。自噬溶酶体的形成是由免疫荧光标记观察LC3B和Lamp1。的表达LC3B、Beclin1 Lamp1被免疫印迹分析。老鼠受到MCAO显示神经评分和恶化细胞缺血性损伤。 These were all significantly reversed by XXMD or CsA. Moreover, XXMD/CsA notably downregulated mitophagy and reduced the increase in LC3, Beclin1, and Lamp1 expression induced by cerebral ischemia and reperfusion. The findings demonstrated that XXMD exerted neuroprotective effect via downregulating LC3, Beclin1, Lamp1, and mitochondrial p62 expression level, thus leading to the inhibition of mitophagy. SN - 0953-4180 UR - https://doi.org/10.1155/2018/4147502 DO - 10.1155/2018/4147502 JF - Behavioural Neurology PB - Hindawi KW - ER -