ty -jour a2 -al -farga，ammar au -yisak，hagos au -tukue，hagos au -redi -abshiro，mesfin au -chandravanshi -chandravanshi，bhagwan singh au -yaya -yaya，estifanos eles ele ele ele ele py -20222 da -20222 da -20222/2022/11/01/01/01 tii- 开发用于直接确定Teff水中总糖的绿色紫外线方法（Eragrostis tef（Zuccagni）trotter）谷物和其他谷物SP -5129510 VL -2022 AB-没有可见的紫外线（UV -VIS）分光光度法，用于直接测定Teff谷物样品水性提取物中总糖的总糖。因此，这项研究的目的是开发一种绿色的紫外线分光光度法，以确定白色Teff，棕色Teff，白米和红麦粒样品的水提取物中的总糖。使用Socrose作为标准标准的校准曲线在20.11–7,907 mg/L的范围内建立 r ² = 0.9996. The limit of detection and limit of quantification were 4.4 and 14.6 mg/L, respectively. The relative standard deviation (6.9%) of the method for the sucrose standard was within the acceptable range indicating that the method is precise. The amount of total sugars determined in the white teff (5.48–9.44% (w/w), brown teff (6.17–10.32% (w/w)), white rice (3.19% (w/w)), and red wheat (9.22% (w/w)) grain samples was comparable with other reported cereal grains. Furthermore, the accuracy of the developed analytical method was also evaluated by spiking the known amount of the sucrose standard solution to the white teff, brown teff, white rice, and red wheat sample extracts, and percentage recoveries found were in the acceptable range (85 ± 2 − 105 ± 4%) with an average recovery of 93%, confirming that the new green method is quantitatively reproducible. Hence, a fast, simple, inexpensive, widely used, selective, sensitive, precise, and accurate green UV-Vis method was developed and validated for the direct determination of total sugars in the aqueous extract of teff, white rice, and red wheat grain samples. SN - 1687-8760 UR - https://doi.org/10.1155/2022/5129510 DO - 10.1155/2022/5129510 JF - International Journal of Analytical Chemistry PB - Hindawi KW - ER -