TY - JOUR A2 - Matias, Ignacio AU - Williams, Dwight A. AU - Williams, Wraegen A. M. AU - Rhoten, Melissa C. AU - Crawley, Charlene D. AU - Ruder, Suzanne PY - 2011 DA - 2011/10/24 TI - Direct Immunosensor Design Based on the Electrochemical Reduction of 4-((4-Nitrophenyl)ethynyl)benzenethiol Monolayers SP - 609758 VL - 2011 AB - The synthesis and characterization of novel N-arylhydroxylamine-based molecular wires are described for use in the site-directed covalent immobilization of whole IgG antibodies onto gold electrode surfaces. The hydroxylamine, electrochemically generated in situ from reduction of the corresponding nitrobenzene, is stable under a wide range of solution conditions and reacts selectively with carbohydrate away from the antibody-binding site to allow the development of immunosensors with maximal activity. Cyclic voltammetric responses have shown a direct correlation between the structure and length of the molecular wire and its stability and concentration at the electrode surface. SN - 1687-725X UR - https://doi.org/10.1155/2011/609758 DO - 10.1155/2011/609758 JF - Journal of Sensors PB - Hindawi Publishing Corporation KW - ER -