TY - JOUR A2 - Pouliot, Marc AU - Nascimento, C. R. AU - Valente, R. C. AU - Echevarria-Lima, J. AU - Fontes, C. F. L. AU - de Araujo-Martins, L. AU - Araujo, E. G. AU - Rumjanek, V. M. PY - 2014 DA - 2014/12/28 TI - The Influence of Ouabain on Human Dendritic Cells Maturation SP - 494956 VL - 2014 AB - Although known as a Na,K-ATPase inhibitor, several other cellular and systemic actions have been ascribed to the steroid Ouabain (Oua). Particularly in the immune system, our group showed that Ouabain acts on decreasing lymphocyte proliferation, synergizing with glucocorticoids in spontaneous thymocyte apoptosis, and also lessening CD14 expression and blocking CD16 upregulation on human monocytes. However, Ouabain effects on dendritic cells (DCs) were not explored so far. Considering the peculiar plasticity and the importance of DCs in immune responses, the aim of our study was to investigate DC maturation under Ouabain influence. To generate immature DCs, human monocytes were cultured with IL-4 and GM-CSF (5 days). To investigate Ouabain role on DC activation, DCs were stimulated with TNF- α.在存在或缺乏瓦巴恩的情况下48小时。在与100nm Ouabain孵育的DC中消除了TNF诱导的CD83表达和IL-12产生，尽管关于淋巴细胞活化的直流功能能力保持不变。尽管如此，TNF- α.- 诱导的抗原捕获下调，另一个成熟标志物，即使在瓦巴因存在下也发生。此外，Oubabain增加了HLA-DR和CD86表达，而CD80表达得到维持。统称，我们的结果表明，DCS响应奥巴宾效率达到一个明显的类别，可能导致免疫和耐受性之间的平衡。SN - 0962-9351 UR - https://doi.org/10.1155/2014/494956 Do - 10.1155/2014 / 494956 JF - 炎症PB的介质 - Hindwai Publishing Corporation KW - ER -