TY -非盟的刘选手盟——徐,野风盟-肖,冯盟——张,剑锋AU -王,以非盟——姚明,YongWei AU -杨,JieWen PY - 2020 DA - 2020/08/01 TI - circRNA-miRNA-mRNA网络的综合分析,揭示潜在的炎症相关的目标为胃腺癌SP - 9435608六世- 2020 AB -胃癌(GC)是最常见的恶性肿瘤的胃。本研究旨在阐明circRNA-miRNA-mRNA的监管网络,确定精确的在GC炎症相关的目标。GSE83521的表达谱,GSE78091, GSE33651获得地理数据库。microrna之间的相互作用和圆形RNA Interactome circRNAs进行调查,并与miRTarBase microrna的目标进行了展望。然后,circRNA / microrna的mRNA的监管网络。另外,选择的差异表达基因的功能富集分析(度)。——炎症/ GC-related GeneCards目标是收集和GenLiP3数据库,分别。和蛋白质相互作用(PPI) DE mrna网络构造字符串和Cytoscape识别中心的基因。基因改变,邻近基因网络,表达水平,和不良预后的基因研究中心cBioPortal, Oncomine,分别和人类的蛋白质图谱数据库和kaplan meier绘图仪。总共有10 DE microrna和33度。 The regulatory network contained 26 circRNAs, 10 miRNAs, and 1459 mRNAs. Functional enrichment analysis revealed that the selected 33 DEGs were involved in negative regulation of fat cell differentiation, response to wounding, extracellular matrix- (ECM-) receptor interaction, and regulation of cell growth pathways. THBS1, FN1, CALM1, COL4A1, CTGF, and IGFBP5 were selected as inflammation-related hub genes of GC in the PPI network. The genetic alterations in these hub genes were related to amplification and missense mutations. Furthermore, the genes RYR2, ERBB2, PI3KCA, and HELZ2 were connected to hub genes in this study. The hub gene levels in clinical specimens were markedly upregulated in GC tissues and correlated with poor overall survival (OS). Our results suggest that THBS1, FN1, CALM1, COL4A1, CTGF, and IGFBP5 were associated with the pathogenesis of gastric carcinogenesis and may serve as biomarkers and inflammation-related targets for GC. SN - 0962-9351 UR - https://doi.org/10.1155/2020/9435608 DO - 10.1155/2020/9435608 JF - Mediators of Inflammation PB - Hindawi KW - ER -