ty -jour a2 -tsokos，玛丽亚·艾（Maria au） - 帕特尔（Patel），尼古尔（Nikul Au） - 布莱克（Nikul au），詹妮弗·阿（Jennifer Au） - 陈（Jennifer Au），xi au -marcondes，A。Marioau -Grady -Grady -Grady，William M. Au -M. Au -Lawlor，Elizabeth R. Au -Borinstein，Borinstein，Scott CCOTT CCOTT CCOTT COTT。PY - 2012 DA - 2012/09/12 TI - DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation SP - 498472 VL - 2012 AB - The role of aberrant DNA methylation in Ewing sarcoma is not completely understood. The methylation status of 503 genes in 52 formalin-fixed paraffin-embedded EWS tumors and 3 EWS cell lines was compared to human mesenchymal stem cell primary cultures (hMSCs) using bead chip methylation analysis. Relative expression of methylated genes was assessed in 5-Aza-2-deoxycytidine-(5-AZA)-treated EWS cell lines and in a cohort of primary EWS samples and hMSCs by gene expression and quantitative RT-PCR. 129 genes demonstrated statistically significant hypermethylation in EWS tumors compared to hMSCs. Thirty-six genes were profoundly methylated in EWS and unmethylated in hMSCs. 5-AZA treatment of EWS cell lines resulted in upregulation of expression of hundreds of genes including 162 that were increased by at least 2-fold. The expression of 19 of 36 candidate hypermethylated genes was increased following 5-AZA. Analysis of gene expression from an independent cohort of tumors confirmed decreased expression of six of nineteen hypermethylated genes ( AXL，COL1A1，CYP1B1，LYN，SERPINE1，） 和 VCAN。比较基因表达和DNA甲基化分析被证明是鉴定EWS表观遗传调节的基因的有效方法。正在进行进一步的研究，以阐明这些表观遗传改变在EWS发病机理中的作用。SN -1357-714X UR -https：//doi.org/10.1155/2012/498472 do -10.1155/2012/2012/498472 JF -Sarcoma PB- Hindawi Publishing Corporation KW- ER-